8AD1
RNA polymerase at U-rich pause bound to RNA putL triple mutant - pause prone, closed clamp state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (N) | Non-template DNA | polymer | 266 | 82140.4 | 1 | Escherichia coli K-12 | ||
| 2 | B (R) | RNA putL triple mutant | polymer | 93 | 29999.8 | 1 | GenBank (1877730557) | Escherichia coli K-12 | |
| 3 | C (T) | Template DNA | polymer | 266 | 82141.5 | 1 | Escherichia coli K-12 | ||
| 4 | D (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | E (F) | RNA polymerase sigma factor RpoD | polymer | 613 | 70327.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli K-12 | Sigma-70 |
| 6 | F, G (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 7 | H (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 8 | I (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1406 | 155237.7 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 9 | J (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
R: 1 - 93 (GenBank: 1877730557)
F: 1 - 613 (UniProt: P00579)
| PDB | External Database | Details |
|---|---|---|
| C 14 | G 3073592 | engineered mutation |
| U 37 | A 3073615 | engineered mutation |
| C 40 | G 3073618 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His 571 | Tyr 571 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 654034.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 654189.5 |
