8AC1

RNA polymerase at U-rich pause bound to non-regulatory RNA - inactive, open clamp state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli K-12	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli K-12	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1406	155237.7	1	UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB	Escherichia coli K-12	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli K-12	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	N	DNA Non-template strand	polymer	295	91015.1	1		Escherichia coli
6	R	Non-regulatory RNA	polymer	122	39226.3	1		Escherichia coli
7	T	DNA Template strand	polymer	295	91180.2	1		Escherichia coli
8	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	610847.1
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	611002.2

*Water molecules are not included.