8AAS

Crystal structure of the Pyrococcus abyssi RPA trimerization core bound to poly-dT20 ssDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Replication factor A	polymer	295	33756.7	1	UniProt (G8ZHS0) Pfam (PF01336) Pfam (PF08646) In PDB	Pyrococcus abyssi GE5
2	B	RPA32 subunit of the hetero-oligomeric complex involved in homologous recombination	polymer	183	21277.9	1	UniProt (Q9V1Z1) Pfam (PF01336) In PDB	Pyrococcus abyssi GE5
3	C	RPA14 subunit of the hetero-oligomeric complex involved in homologous recombination	polymer	122	14008.9	1	UniProt (Q9V1Z0) In PDB	Pyrococcus abyssi GE5
4	F	DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')	polymer	20	6038.9	1		synthetic construct
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	A, B, C	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)

Sequence modifications

B: 2 - 182 (UniProt: Q9V1Z1)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag

C: 2 - 117 (UniProt: Q9V1Z0)

PDB	External Database	Details
Gly -4	-	expression tag
Thr -3	-	expression tag
Gly -2	-	expression tag
Asp -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	75082.4
Non-Polymers*	Number of molecules	6
	Total formula weight	265.8
All*	Total formula weight	75348.2

*Water molecules are not included.