8AAS
Crystal structure of the Pyrococcus abyssi RPA trimerization core bound to poly-dT20 ssDNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Replication factor A | polymer | 295 | 33756.7 | 1 | UniProt (G8ZHS0) | Pyrococcus abyssi GE5 | |
| 2 | B (B) | RPA32 subunit of the hetero-oligomeric complex involved in homologous recombination | polymer | 183 | 21277.9 | 1 | UniProt (Q9V1Z1) | Pyrococcus abyssi GE5 | |
| 3 | C (C) | RPA14 subunit of the hetero-oligomeric complex involved in homologous recombination | polymer | 122 | 14008.9 | 1 | UniProt (Q9V1Z0) | Pyrococcus abyssi GE5 | |
| 4 | D (F) | DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') | polymer | 20 | 6038.9 | 1 | synthetic construct | ||
| 5 | E (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | F, G, H, I, J (A, B, C) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) |
Sequence modifications
B: 2 - 182 (UniProt: Q9V1Z1)
C: 2 - 117 (UniProt: Q9V1Z0)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Thr -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 75082.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 265.8 | |
| All* | Total formula weight | 75348.2 |
