8A9Q
Computational design of stable mammalian serum albumins for bacterial expression
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Albumin | polymer | 585 | 66729.4 | 2 | UniProt (P02768) Pfam (PF00273) | Escherichia coli | |
| 2 | C, D, E, F, H... (A, B) | MYRISTIC ACID | non-polymer | 228.4 | 7 | Chemie (MYR) | |||
| 3 | G, L (A, B) | R-WARFARIN | non-polymer | 308.3 | 2 | Chemie (RWF) | |||
| 4 | J (B) | LAURIC ACID | non-polymer | 200.3 | 1 | Chemie (DAO) | |||
| 5 | M, N (A, B) | water | water | 18.0 | 91 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 585 (UniProt: P02768)
| PDB | External Database | Details |
|---|---|---|
| Leu 39 | His 63 | engineered mutation |
| Met 42 | Leu 66 | engineered mutation |
| Pro 120 | Val 144 | engineered mutation |
| Tyr 156 | Phe 180 | engineered mutation |
| Glu 187 | Asp 211 | engineered mutation |
| His 198 | Leu 222 | engineered mutation |
| Ile 202 | Ser 226 | engineered mutation |
| Ile 310 | Val 334 | engineered mutation |
| Ser 371 | Ala 395 | engineered mutation |
| Ile 381 | Val 405 | engineered mutation |
| Ile 409 | Val 433 | engineered mutation |
| Ala 427 | Ser 451 | engineered mutation |
| Ile 455 | Val 479 | engineered mutation |
| Glu 519 | Lys 543 | engineered mutation |
| Ser 552 | Ala 576 | engineered mutation |
| Ile 576 | Val 600 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 133458.8 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2415.6 | |
| All* | Total formula weight | 135874.4 |
