8A7H

Crystal structure of a chimeric LOV-Histidine kinase SB2F1-I66R mutant (light state; asymmetrical variant, trigonal form with long c axis)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putative Sensory box protein,Sensor protein FixL	polymer	388	42944.0	2	UniProt (Q88JB0) UniProt (P23222) Pfam (PF13426) Pfam (PF00512) Pfam (PF02518)	Pseudomonas putida KT2440
2	C, E (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	D, F (A, B)	Flavin mononucleotide (semi-quinone intermediate)	non-polymer		458.4	2	Chemie (JGC)

Sequence modifications

A, B: 1 - 120 (UniProt: Q88JB0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 66	Ile 69	engineered mutation

A, B: 121 - 368 (UniProt: P23222)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85888.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1931.1
All*	Total formula weight	87819.1

*Water molecules are not included.