8A53

Crystal structure of AtMCA-IIf C147A (metacaspase 9) from Arabidopsis thaliana

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Metacaspase-9	polymer	348	38375.5	4	UniProt (Q9FYE1) Pfam (PF00656)	Arabidopsis thaliana (thale cress)	AtMC9
2	E, F, G (A, B)	NITRATE ION	non-polymer		62.0	3	Chemie (NO3)
3	H, I, J, K (A, B, C, D)	water	water		18.0	416	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 325 (UniProt: Q9FYE1)

PDB	External Database	Details
Met -22	-	initiating methionine
Ser -21	-	expression tag
Tyr -20	-	expression tag
Tyr -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Leu -12	-	expression tag
Glu -11	-	expression tag
Ser -10	-	expression tag
Thr -9	-	expression tag
Ser -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Lys -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Thr 0	-	expression tag
Arg 5	Gly 5	cloning artifact
Ala 147	Cys 147	engineered mutation
Tyr 271	Phe 271	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	153502.2
Non-Polymers*	Number of molecules	3
	Total formula weight	186.0
All*	Total formula weight	153688.2

*Water molecules are not included.