8A37

Crystal structure of PpSB1-LOV-K117E mutant (dark state), tetragonal form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sensory box protein	polymer	162	18617.8	1	UniProt (Q88E39) Pfam (PF13426) In PDB	Pseudomonas putida KT2440
2	A	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4		water	water		18.0	87	Chemie (HOH)

Sequence modifications

A: 1 - 142 (UniProt: Q88E39)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Glu 117	Lys 117	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18617.8
Non-Polymers*	Number of molecules	2
	Total formula weight	562.5
All*	Total formula weight	19180.2

*Water molecules are not included.