7ZZ8

Cryo-EM structure of Lactococcus lactis pyruvate carboxylase with acetyl-CoA and cyclic di-AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Pyruvate carboxylase	polymer	1143	127349.6	4	UniProt (A0A2A9IR05) In PDB	Lactococcus lactis
2	D, A, B, C	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
3	A, B, C, D	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
4	D, A, B, C	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
5	D, A, B, C	ACETYL COENZYME *A	non-polymer		809.6	4	Chemie (ACO)
6	D, A, B, C	PYRUVIC ACID	non-polymer		88.1	4	Chemie (PYR)
7	A, C	(2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8 ]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide	non-polymer		658.4	2	Chemie (2BA)
8		water	water		18.0	4	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 1137 (UniProt: A0A2A9IR05)

PDB	External Database	Details
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 1055	Thr 1055	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	509398.3
Non-Polymers*	Number of molecules	26
	Total formula weight	7030.4
All*	Total formula weight	516428.7

*Water molecules are not included.