7ZZ3

Cryo-EM structure of "BC react" conformation of Lactococcus lactis pyruvate carboxylase with acetyl-CoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Pyruvate carboxylase	polymer	1143	127349.6	4	UniProt (A0A2A9IR05) In PDB	Lactococcus lactis
2	A	BIOTIN	non-polymer		244.3	1	Chemie (BTN)
3	D, A, B, C	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
4	A, B, C, D	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
5	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
6	D, A, B, C	ACETYL COENZYME *A	non-polymer		809.6	4	Chemie (ACO)
7	D, A, B, C	PYRUVIC ACID	non-polymer		88.1	4	Chemie (PYR)
8	A	BICARBONATE ION	non-polymer		61.0	1	Chemie (BCT)
9	D, B, C	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	3	Chemie (ADP)
10		water	water		18.0	166	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 1137 (UniProt: A0A2A9IR05)

PDB	External Database	Details
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 1055	Thr 1055	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	509398.3
Non-Polymers*	Number of molecules	26
	Total formula weight	6098.8
All*	Total formula weight	515497.1

*Water molecules are not included.