7ZYT

Crystal structure of the I318T pathogenic variant of the human dihydrolipoamide dehydrogenase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Dihydrolipoyl dehydrogenase, mitochondrial	polymer	496	52625.1	2	UniProt (P09622) Pfam (PF07992) Pfam (PF02852) In PDB	Homo sapiens (human)	Dihydrolipoamide dehydrogenase,Glycine cleavage system L protein
2	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	A, B	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	2	Chemie (BTB)
4	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)

Sequence modifications

A, B: 1 - 474 (UniProt: P09622)

PDB	External Database	Details
Met -21	-	initiating methionine
Ala -20	-	expression tag
Ser -19	-	expression tag
Trp -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Pro -15	-	expression tag
Gln -14	-	expression tag
Phe -13	-	expression tag
Glu -12	-	expression tag
Lys -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Thr 318	Ile 353	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	105250.2
Non-Polymers*	Number of molecules	5
	Total formula weight	2051.6
All*	Total formula weight	107301.9

*Water molecules are not included.