7ZV9

Crystal structure of FLT3 in complex with a monomeric FLT3 Ligand variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	Fms-related tyrosine kinase 3 ligand	polymer	155	17764.2	8	UniProt (P49771) Pfam (PF02947) In PDB	Homo sapiens (human)	Flt3 ligand,Flt3L,SL cytokine
2	B, D, F, H, J...	Receptor-type tyrosine-protein kinase FLT3	polymer	582	65307.8	8	UniProt (P36888) Pfam (PF00047) In PDB	Homo sapiens (human)	FL cytokine receptor,Fetal liver kinase-2,FLK-2,Fms-like tyrosine kinase 3,FLT-3,Stem cell tyrosine kinase 1,STK-1
3	Q, R, S, T, U...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
4	B, H, P	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, C, E, G, I, K, M, O: 1 - 134 (UniProt: P49771)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Asp 27	Leu 53	engineered mutation

B, D, F, H, J, L, N, P: 1 - 541 (UniProt: P36888)

PDB	External Database	Details
Met 227	Thr 227	conflict
Gly 542	-	expression tag
Ser 543	-	expression tag
Ser 544	-	expression tag
Gly 545	-	expression tag
Leu 546	-	expression tag
Val 547	-	expression tag
Pro 548	-	expression tag
Arg 549	-	expression tag
Gly 550	-	expression tag
Ser 551	-	expression tag
Gly 552	-	expression tag
Gly 553	-	expression tag
Ser 554	-	expression tag
Gly 555	-	expression tag
Gly 556	-	expression tag
Ser 557	-	expression tag
Gly 558	-	expression tag
Leu 559	-	expression tag
Asn 560	-	expression tag
Asp 561	-	expression tag
Ile 562	-	expression tag
Phe 563	-	expression tag
Glu 564	-	expression tag
Ala 565	-	expression tag
Gln 566	-	expression tag
Lys 567	-	expression tag
Ile 568	-	expression tag
Glu 569	-	expression tag
Trp 570	-	expression tag
His 571	-	expression tag
Glu 572	-	expression tag
Gly 573	-	expression tag
Arg 574	-	expression tag
Thr 575	-	expression tag
Lys 576	-	expression tag
His 577	-	expression tag
His 578	-	expression tag
His 579	-	expression tag
His 580	-	expression tag
His 581	-	expression tag
His 582	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	664576.5
Branched	Number of molecules	6
	Total formula weight	2546.4
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	668007.8

*Water molecules are not included.