7ZRM

Cryo-EM map of the unphosphorylated KdpFABC complex in the E1-P_ADP conformation, under turnover conditions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Potassium-transporting ATPase potassium-binding subunit	polymer	557	59218.6	1	UniProt (P03959) Pfam (PF03814)	Escherichia coli	ATP phosphohydrolase [potassium-transporting] A chain,Potassium-binding and translocating subunit A,Potassium-translocating ATPase A chain
2	B (C)	Potassium-transporting ATPase KdpC subunit	polymer	190	20281.0	1	UniProt (P03961) Pfam (PF02669)	Escherichia coli	ATP phosphohydrolase [potassium-transporting] C chain,Potassium-binding and translocating subunit C,Potassium-translocating ATPase C chain
3	C (D)	Potassium-transporting ATPase KdpF subunit	polymer	27	2853.5	1	UniProt (P36937) Pfam (PF09604)	Escherichia coli	ATP phosphohydrolase [potassium-transporting] F chain,Potassium-binding and translocating subunit F,Potassium-translocating ATPase F chain
4	D (B)	Potassium-transporting ATPase ATP-binding subunit	polymer	682	72331.8	1	UniProt (P03960) Pfam (PF00122) Pfam (PF00702)	Escherichia coli	ATP phosphohydrolase [potassium-transporting] B chain,Potassium-binding and translocating subunit B,Potassium-translocating ATPase B chain
5	E, F, G, H, I (A)	POTASSIUM ION	non-polymer		39.1	5	Chemie (K)
6	J (B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
7	K (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

B: 1 - 682 (UniProt: P03960)

PDB	External Database	Details
Ala 162	Ser 162	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	154685.0
Non-Polymers*	Number of molecules	7
	Total formula weight	647.0
All*	Total formula weight	155332.0

*Water molecules are not included.