7ZR2

Crystal structure of a chimeric protein mimic of SARS-CoV-2 Spike HR1 in complex with HR2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Spike protein S2',Chimeric protein mimic of SARS-CoV-2 Spike HR1	polymer	241	26577.7	1	UniProt (P0DTC2)	Severe acute respiratory syndrome coronavirus 2
2	B (B)	Spike protein S2'	polymer	44	4673.1	1	UniProt (P0DTC2)	Severe acute respiratory syndrome coronavirus 2
3	C, D (A, B)	water	water		18.0	304	Chemie (HOH)

Sequence modifications

A: 2 - 75 (UniProt: P0DTC2)

PDB	External Database	Details
Met 0	-	initiating methionine
Asp 1	-	expression tag
Lys 13	Gln 926	engineered mutation
Glu 31	Ala 944	engineered mutation
Glu 38	Val 951	engineered mutation
Asp 45	Ala 958	engineered mutation
Arg 59	Ala 972	engineered mutation
Glu 63	Val 976	engineered mutation
Gly 74	Val 987	engineered mutation

A: 156 - 229 (UniProt: P0DTC2)

PDB	External Database	Details
Pro 76	-	linker
Ala 77	-	linker
Lys 78	-	linker
Asp 79	-	linker
Leu 80	-	linker
Arg 81	-	linker
Ser 82	-	linker
Asp 83	-	linker
Ile 84	-	linker
Asp 85	-	linker
Asn 86	-	linker
Leu 87	-	linker
Glu 88	-	linker
Ser 89	-	linker
Lys 90	-	linker
Ile 91	-	linker
Ala 92	-	linker
Gly 93	-	linker
Phe 94	-	linker
Asn 95	-	linker
Ser 96	-	linker
Ser 97	-	linker
Leu 98	-	linker
Gln 99	-	linker
Lys 100	-	linker
Val 101	-	linker
Leu 102	-	linker
Thr 103	-	linker
Asn 104	-	linker
Leu 105	-	linker
Ala 106	-	linker
Gln 107	-	linker
Lys 108	-	linker
Asn 109	-	linker
Gln 110	-	linker
Asn 111	-	linker
Val 112	-	linker
Glu 113	-	linker
Asp 114	-	linker
Lys 115	-	linker
Leu 116	-	linker
Lys 117	-	linker
Gly 118	-	linker
Leu 119	-	linker
Glu 120	-	linker
Ser 121	-	linker
Arg 122	-	linker
Thr 123	-	linker
Ser 124	-	linker
Ser 125	-	linker
Leu 126	-	linker
Glu 127	-	linker
Lys 128	-	linker
Gln 129	-	linker
Ile 130	-	linker
Lys 131	-	linker
Gly 132	-	linker
Ile 133	-	linker
Ala 134	-	linker
Ser 135	-	linker
Asn 136	-	linker
Phe 137	-	linker
Gln 138	-	linker
Asn 139	-	linker
Glu 140	-	linker
Ile 141	-	linker
Leu 142	-	linker
Lys 143	-	linker
Gln 144	-	linker
Arg 145	-	linker
Glu 146	-	linker
Tyr 147	-	linker
Leu 148	-	linker
Val 149	-	linker
Asn 150	-	linker
Lys 151	-	linker
Gly 152	-	linker
Ser 153	-	linker
Gly 154	-	linker
Asn 155	-	linker
Glu 165	Ala 924	engineered mutation
Lys 183	Ala 942	engineered mutation
Lys 190	Gln 949	engineered mutation
Lys 197	Ala 956	engineered mutation
Lys 222	Leu 981	engineered mutation
Gly 230	-	expression tag
Gly 231	-	expression tag
Gly 232	-	expression tag
Gly 233	-	expression tag
Ser 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag

B: 1164 - 1202 (UniProt: P0DTC2)

PDB	External Database	Details
Ace 1163	-	acetylation
Ser 1203	-	expression tag
Gly 1204	-	expression tag
Gly 1205	-	expression tag
Tyr 1206	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31250.8
All*	Total formula weight	31250.8

*Water molecules are not included.