7ZOF

Streptavidin Iron-Porphyrin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Streptavidin	polymer	158	16364.7	4	UniProt (P22629) Pfam (PF01382)	Streptomyces avidinii
2	E, G (A, B)	(2R)-2-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-3-[[(5Z,10Z,14Z,19Z)-15-[[[(2R)-2-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-3-sulfo-propanoyl]amino]methyl]-1,4,21,23-tetrahydroporphyrin-5-yl]methylamino]-3-oxidanylidene-propane-1-sulfonic acid	non-polymer		1125.3	2	Chemie (JLL)
3	F, H, I, K (A, B, C, D)	IMIDAZOLE	non-polymer		69.1	4	Chemie (IMD)
4	J, L (C, D)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
5	M, N, O, P (A, B, C, D)	water	water		18.0	139	Chemie (HOH)

Sequence modifications

A, B, C, D: 14 - 159 (UniProt: P22629)

PDB	External Database	Details
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Ala 112	Ser 136	engineered mutation
Ala 121	Lys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65458.9
Non-Polymers*	Number of molecules	8
	Total formula weight	2638.7
All*	Total formula weight	68097.6

*Water molecules are not included.