7ZO1
SpCas9 bound to CD34 off-target9 DNA substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CD34 sgRNA | polymer | 85 | 27598.5 | 1 | synthetic construct | ||
| 2 | B (B) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 3 | C (C) | CD34 off-target9 DNA target strand | polymer | 28 | 8394.4 | 1 | synthetic construct | ||
| 4 | D (D) | CD34 off-target9 DNA non-target strand | polymer | 12 | 3716.5 | 1 | synthetic construct | ||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G, H, I, J, K... (A, B) | POTASSIUM ION | non-polymer | 39.1 | 8 | Chemie (K) | |||
| 7 | O, P, Q, R (A, B, C, D) | water | water | 18.0 | 284 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 198298.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 361.4 | |
| All* | Total formula weight | 198659.5 |
