7ZK4

The ABCB1 L335C mutant (mABCB1) in the outward facing state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ATP-dependent translocase ABCB1	polymer	1328	146063.9	1	UniProt (P21447) Pfam (PF00664) Pfam (PF00005)	Mus musculus (house mouse)	ATP-binding cassette sub-family B member 1A,MDR1A,Multidrug resistance protein 1A,Multidrug resistance protein 3,P-glycoprotein 3,Phospholipid transporter ABCB1
2	B, D (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	C, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, G, H, I, J (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	5	Chemie (Y01)
5	K (A)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

A: 1 - 1276 (UniProt: P21447)

PDB	External Database	Details
Gln 83	Asn 83	engineered mutation
Gln 87	Asn 87	engineered mutation
Gln 90	Asn 90	engineered mutation
Ala 133	Cys 133	engineered mutation
Cys 335	Leu 335	engineered mutation
Gly 427	Cys 427	engineered mutation
Gly 638	Cys 638	engineered mutation
Thr 669	Cys 669	engineered mutation
Ala 713	Cys 713	engineered mutation
Ala 952	Cys 952	engineered mutation
Gly 1070	Cys 1070	engineered mutation
Arg 1121	Cys 1121	engineered mutation
Val 1223	Cys 1223	engineered mutation
Leu 1277	-	expression tag
Glu 1278	-	expression tag
Glu 1279	-	expression tag
Asn 1280	-	expression tag
Leu 1281	-	expression tag
Tyr 1282	-	expression tag
Phe 1283	-	expression tag
Gln 1284	-	expression tag
Gly 1285	-	expression tag
Gly 1286	-	expression tag
Gly 1287	-	expression tag
Ala 1288	-	expression tag
Ser 1289	-	expression tag
Gly 1290	-	expression tag
Gly 1291	-	expression tag
Ser 1292	-	expression tag
Trp 1293	-	expression tag
Ser 1294	-	expression tag
His 1295	-	expression tag
Pro 1296	-	expression tag
Gln 1297	-	expression tag
Phe 1298	-	expression tag
Glu 1299	-	expression tag
Lys 1300	-	expression tag
Ala 1301	-	expression tag
Ala 1302	-	expression tag
Ala 1303	-	expression tag
Gly 1304	-	expression tag
Gly 1305	-	expression tag
Gly 1306	-	expression tag
Ser 1307	-	expression tag
Gly 1308	-	expression tag
Gly 1309	-	expression tag
Gly 1310	-	expression tag
Ser 1311	-	expression tag
Trp 1312	-	expression tag
Ser 1313	-	expression tag
His 1314	-	expression tag
Pro 1315	-	expression tag
Gln 1316	-	expression tag
Phe 1317	-	expression tag
Glu 1318	-	expression tag
Lys 1319	-	expression tag
Gly 1320	-	expression tag
Ser 1321	-	expression tag
Gly 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag
His 1325	-	expression tag
His 1326	-	expression tag
His 1327	-	expression tag
His 1328	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	146063.9
Non-Polymers*	Number of molecules	9
	Total formula weight	3496.6
All*	Total formula weight	149560.5

*Water molecules are not included.