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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

7ZAG

Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA,mRNA, aIF1A and the C-terminal domain of aIF5B.

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
12rRNApolymer1497487993.11GenBank (5457433)Pyrococcus abyssi GE5
2A30S ribosomal protein S3Aepolymer19922888.11UniProt (Q9V2K7)
Pfam (PF01015)
In PDB		Pyrococcus abyssi GE5Ribosomal protein S1e
3B30S ribosomal protein S2polymer20223026.91UniProt (Q9V191)
Pfam (PF00318)
In PDB		Pyrococcus abyssi GE5
4CZn-ribbon RNA-binding protein involved in translationpolymer637163.41UniProt (G8ZFK7)
Pfam (PF07754)
In PDB		Pyrococcus abyssi GE5
5D30S ribosomal protein S4polymer18021381.91UniProt (P61992)
Pfam (PF01479)
In PDB		Pyrococcus abyssi GE5
6E30S ribosomal protein S4epolymer24328246.11UniProt (Q9V1U8)
Pfam (PF08071)
Pfam (PF01479)
Pfam (PF00900)
In PDB		Pyrococcus abyssi GE5
7F30S ribosomal protein S5polymer23626523.91UniProt (Q9V1V5)
Pfam (PF00333)
Pfam (PF03719)
In PDB		Pyrococcus abyssi GE5
8G30S ribosomal protein S6epolymer12514029.51UniProt (Q9UYS3)
Pfam (PF01092)
In PDB		Pyrococcus abyssi GE5
9H30S ribosomal protein S7polymer21524662.81UniProt (Q9V109)
Pfam (PF00177)
In PDB		Pyrococcus abyssi GE5
10I30S ribosomal protein S8polymer13014772.21UniProt (Q9V1V0)
Pfam (PF00410)
In PDB		Pyrococcus abyssi GE5
11J30S ribosomal protein S8epolymer12714293.71UniProt (Q9UZL4)
Pfam (PF01201)
In PDB		Pyrococcus abyssi GE5
12K30S ribosomal protein S9polymer13515293.91UniProt (Q9V195)
Pfam (PF00380)
In PDB		Pyrococcus abyssi GE5
13L30S ribosomal protein S10polymer10211795.81UniProt (Q9V0V6)
Pfam (PF00338)
In PDB		Pyrococcus abyssi GE5
14M30S ribosomal protein S11polymer13714773.01UniProt (P62010)
Pfam (PF00411)
In PDB		Pyrococcus abyssi GE5
15N30S ribosomal protein S12polymer14716477.61UniProt (P61994)
Pfam (PF00164)
In PDB		Pyrococcus abyssi GE5
16O30S ribosomal protein S13polymer14816992.91UniProt (Q9V1A0)
Pfam (PF00416)
In PDB		Pyrococcus abyssi GE5
17P30S ribosomal protein S14 type Zpolymer566644.11UniProt (P62012)
Pfam (PF00253)
In PDB		Pyrococcus abyssi GE5
18Q30S ribosomal protein S15polymer15818697.11UniProt (Q9V2K9)
Pfam (PF08069)
Pfam (PF00312)
In PDB		Pyrococcus abyssi GE5
19R30S ribosomal protein S17polymer11313364.61UniProt (Q9V1U5)
Pfam (PF00366)
In PDB		Pyrococcus abyssi GE5
20S30S ribosomal protein S17epolymer678059.51UniProt (Q9V0G0)
Pfam (PF00833)
In PDB		Pyrococcus abyssi GE5
21T30S ribosomal protein S19polymer13215307.31UniProt (Q9V1T9)
Pfam (PF00203)
In PDB		Pyrococcus abyssi GE5
22U30S ribosomal protein S19epolymer15017422.31UniProt (Q9V0G8)
Pfam (PF01090)
In PDB		Pyrococcus abyssi GE5
23V30S ribosomal protein S24epolymer9911798.61UniProt (Q9UY20)
Pfam (PF01282)
In PDB		Pyrococcus abyssi GE5
24W30S ribosomal protein S27epolymer657186.61UniProt (Q9UXZ3)
Pfam (PF01667)
In PDB		Pyrococcus abyssi GE5
25X30S ribosomal protein S28epolymer718102.31UniProt (P61029)
Pfam (PF01200)
In PDB		Pyrococcus abyssi GE5
26Y30S ribosomal protein S27aepolymer515992.21UniProt (P61238)
Pfam (PF01599)
In PDB		Pyrococcus abyssi GE5
27Z30S ribosomal protein S3polymer21023472.31UniProt (Q9V1U1)
Pfam (PF07650)
Pfam (PF00189)
In PDB		Pyrococcus abyssi GE5
28050S ribosomal protein L41epolymer364910.21UniProt (Q8U232)
In PDB		Pyrococcus abyssi GE5Large ribosomal subunit protein eL41
29350S ribosomal protein L7Aepolymer12313442.71UniProt (P62008)
Pfam (PF01248)
In PDB		Pyrococcus abyssi GE5Ribosomal protein L8e
305mRNApolymer258064.81Pyrococcus abyssi GE5
314Met-tRNAiMETpolymer7724833.91GenBank (1334604293)Pyrococcus abyssi GE5
326Translation initiation factor 1Apolymer13415336.71UniProt (Q9V138)
Pfam (PF01176)
In PDB		Pyrococcus abyssi GE5aIF-1A
337Translation initiation factor 5Bpolymer61969122.91UniProt (Q9UZK7)
Pfam (PF00009)
Pfam (PF03144)
Pfam (PF11987)
Pfam (PF14578)
In PDB		Pyrococcus abyssi GE5
342, F, K, 5, 4MAGNESIUM IONnon-polymer24.362Chemie (MG)
35C, F, P, R, WZINC IONnon-polymer65.46Chemie (ZN)
364METHIONINEnon-polymer149.21Chemie (MET)
37waterwater18.0379Chemie (HOH)
Sequence modifications
2: 13 - 1509 (GenBank: 5457433)
PDBExternal DatabaseDetails
4ac 17C 205055modified residue
4ac 53C 205091modified residue
4ac 286C 205324modified residue
4ac 303C 205341modified residue
4ac 319C 205357modified residue
4ac 379C 205417modified residue
4ac 394C 205432modified residue
4ac 479C 205517modified residue
4ac 511C 205549modified residue
4ac 546C 205584modified residue
4ac 590C 205628modified residue
4ac 626C 205664modified residue
4ac 636C 205674modified residue
4ac 703C 205741modified residue
4ac 718C 205756modified residue
4ac 731C 205769modified residue
4ac 751C 205789modified residue
4ac 828C 205866modified residue
4ac 839C 205877modified residue
4ac 848C 205886modified residue
4ac 851C 205889modified residue
4ac 868C 205906modified residue
4ac 957C 205995modified residue
4ac 1028C 206066modified residue
4ac 1147C 206185modified residue
4ac 1184C 206222modified residue
4ac 1233C 206271modified residue
4ac 1239C 206277modified residue
4ac 1479C 206517modified residue
4: 1 - 76 (GenBank: 1334604293)
PDBExternal DatabaseDetails
A 1C 3301604engineered mutation
U 72A 3301532engineered mutation
6: -20 - 0 (PDB: 7ZAG)
6: 1 - 113 (UniProt: Q9V138)
7: 1 - 20 (UniProt: Q9UZK7)
PDBExternal DatabaseDetails
Met -20-initiating methionine
Gly -19-expression tag
Ser -18-expression tag
Ser -17-expression tag
His -16-expression tag
His -15-expression tag
His -14-expression tag
His -13-expression tag
His -12-expression tag
His -11-expression tag
Ser -10-expression tag
Ser -9-expression tag
Gly -8-expression tag
Leu -7-expression tag
Val -6-expression tag
Pro -5-expression tag
Arg -4-expression tag
Gly -3-expression tag
Ser -2-expression tag
His -1-expression tag
Met 0-expression tag
7: 21 - 598 (UniProt: Q9UZK7)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains33
Total formula weight1032072.9
Non-Polymers*Number of molecules69
Total formula weight2048.6
All*Total formula weight1034121.5
*Water molecules are not included.

219869

PDB entries from 2024-05-15

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