7Z8N

GacS histidine kinase from Pseudomonas aeruginosa

Replaces:  6ZLS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Histidine kinase	polymer	317	35389.4	4	UniProt (G3XD98) Pfam (PF00672) Pfam (PF00512) Pfam (PF02518)	Pseudomonas aeruginosa PAO1
2	E, I, J (A, B)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
3	F, G, H (B)	R-1,2-PROPANEDIOL	non-polymer		76.1	3	Chemie (PGR)
4	K, L, M, N (A, B, C, D)	water	water		18.0	178	Chemie (HOH)

Sequence modifications

A, B, C, D: 220 - 512 (UniProt: G3XD98)

PDB	External Database	Details
Mse 196	-	initiating methionine
Gly 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
His 203	-	expression tag
Ser 204	-	expression tag
Ser 205	-	expression tag
Gly 206	-	expression tag
Val 207	-	expression tag
Asp 208	-	expression tag
Leu 209	-	expression tag
Gly 210	-	expression tag
Thr 211	-	expression tag
Glu 212	-	expression tag
Asn 213	-	expression tag
Leu 214	-	expression tag
Tyr 215	-	expression tag
Phe 216	-	expression tag
Gln 217	-	expression tag
Ser 218	-	expression tag
Mse 219	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	141557.5
Non-Polymers*	Number of molecules	6
	Total formula weight	348.5
All*	Total formula weight	141906.0

*Water molecules are not included.