7Z7H

Structure of P. luminescens TccC3-F-actin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D, E, F (D, B, E, C, A)	Actin, alpha skeletal muscle	polymer	377	42110.0	5	UniProt (P68135) Pfam (PF00022)	Oryctolagus cuniculus (rabbit)	Alpha-actin-1
2	B (F)	Maltose/maltodextrin-binding periplasmic protein,TccC3	polymer	578	63658.9	1	UniProt (P0AEX9) UniProt (Q8GF97) Pfam (PF01547)	Photorhabdus luminescens	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
3	G, K, M, O, Q (D, B, E, C, A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	5	Chemie (ADP)
4	H, L, N, P, R (D, B, E, C, A)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
5	I (D)	ADENOSINE-5-DIPHOSPHORIBOSE	non-polymer		559.3	1	Chemie (APR)
6	J (F)	NICOTINAMIDE	non-polymer		122.1	1	Chemie (NCA)

Sequence modifications

F: -274 - 91 (UniProt: P0AEX9)

PDB	External Database	Details
Met -295	-	initiating methionine
Gly -294	-	expression tag
Ser -293	-	expression tag
Ser -292	-	expression tag
His -291	-	expression tag
His -290	-	expression tag
His -289	-	expression tag
His -288	-	expression tag
His -287	-	expression tag
His -286	-	expression tag
Ser -285	-	expression tag
Ser -284	-	expression tag
Gly -283	-	expression tag
Leu -282	-	expression tag
Val -281	-	expression tag
Pro -280	-	expression tag
Arg -279	-	expression tag
Gly -278	-	expression tag
Ser -277	-	expression tag
His -276	-	expression tag
Met -275	-	expression tag
Val 37	Ala 338	variant
Asn 92	-	linker
Ser 93	-	linker
Gly 94	-	linker
Ser 95	-	linker
Ser 96	-	linker
Gly 97	-	linker
Ser 98	-	linker
Ser 99	-	linker
Gly 100	-	linker

F: 101 - 282 (UniProt: Q8GF97)

PDB	External Database	Details
Ser 265	Glu 943	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	274208.8
Non-Polymers*	Number of molecules	12
	Total formula weight	2939.0
All*	Total formula weight	277147.8

*Water molecules are not included.