7Z5U

Crystal structure of the peptidase domain of collagenase G from Clostridium histolyticum in complex with a hydroxamate-based inhibitor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Collagenase ColG	polymer	415	47885.9	1	UniProt (Q9X721)	Hathewaya histolytica	Class I collagenase,Gelatinase ColG,Microbial collagenase
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	C (A)	(2~{R})-~{N}-[2-[4-[(2-acetamidophenoxy)methyl]-1,2,3-triazol-1-yl]ethyl]-2-(2-methylpropyl)-~{N}'-oxidanyl-propanediamide	non-polymer		432.5	1	Chemie (IFW)
4	D (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	E (A)	water	water		18.0	149	Chemie (HOH)

Sequence modifications

A: 398 - 790 (UniProt: Q9X721)

PDB	External Database	Details
Met 376	-	initiating methionine
Gly 377	-	expression tag
Ser 378	-	expression tag
Ser 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
Ser 386	-	expression tag
Ser 387	-	expression tag
Gly 388	-	expression tag
Glu 389	-	expression tag
Asn 390	-	expression tag
Leu 391	-	expression tag
Tyr 392	-	expression tag
Phe 393	-	expression tag
Gln 394	-	expression tag
Gly 395	-	expression tag
Gly 396	-	expression tag
Thr 397	-	expression tag
Leu 575	Ser 575	engineered mutation
Val 665	Ala 665	engineered mutation
Gly 790	Ala 790	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47885.9
Non-Polymers*	Number of molecules	3
	Total formula weight	538.0
All*	Total formula weight	48423.8

*Water molecules are not included.