7Z19

E. coli C-P lyase bound to a single PhnK ABC domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG	polymer	150	16560.6	2	UniProt (P16685) Pfam (PF06754)	Escherichia coli	RPnTP synthase subunit PhnG
2	B, F (B, F)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH	polymer	194	21074.3	2	UniProt (P16686) Pfam (PF05845)	Escherichia coli	RPnTP synthase subunit PhnH
3	C, G (C, G)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI	polymer	354	38922.7	2	UniProt (P16687) Pfam (PF05861)	Escherichia coli	RPnTP synthase subunit PhnI,Ribose 1-methylphosphonate 5-triphosphate synthase nucleosidase subunit
4	D, H (D, H)	Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase	polymer	281	31879.1	2	UniProt (P16688) Pfam (PF06007)	Escherichia coli	PRPn C-P lyase
5	I (I)	Putative phosphonates utilization ATP-binding protein PhnK	polymer	258	28665.7	1	UniProt (P16678) Pfam (PF00005) Pfam (PF08352)	Escherichia coli
6	J, K, M, N (C, D, G, H)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	L, O (D, H)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
8	P, Q, R, S, T... (A, B, C, D, E...)	water	water		18.0	477	Chemie (HOH)

Sequence modifications

B, F: 1 - 194 (UniProt: P16686)

PDB	External Database	Details
Arg 152	Gln 152	conflict

C, G: 1 - 354 (UniProt: P16687)

PDB	External Database	Details
Val 322	Ala 322	conflict

I: 1 - 252 (UniProt: P16678)

PDB	External Database	Details
Ala 208	Val 208	conflict
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	245539.1
Non-Polymers*	Number of molecules	6
	Total formula weight	451.6
All*	Total formula weight	245990.6

*Water molecules are not included.