7Z15

E. coli C-P lyase bound to a PhnK/PhnL dual ABC dimer and ADP + Pi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG	polymer	150	16545.7	2	UniProt (P16685) Pfam (PF06754)	Escherichia coli	RPnTP synthase subunit PhnG
2	B, F (B, F)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH	polymer	194	21045.2	2	UniProt (P16686) Pfam (PF05845)	Escherichia coli	RPnTP synthase subunit PhnH
3	C, G (C, G)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI	polymer	354	38953.7	2	UniProt (P16687) Pfam (PF05861)	Escherichia coli	RPnTP synthase subunit PhnI,Ribose 1-methylphosphonate 5-triphosphate synthase nucleosidase subunit
4	D, H (D, H)	Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase	polymer	281	31893.1	2	UniProt (P16688) Pfam (PF06007)	Escherichia coli	PRPn C-P lyase
5	I, J (I, J)	Putative phosphonates utilization ATP-binding protein PhnK	polymer	291	31995.1	2	UniProt (P16678) Pfam (PF00005) Pfam (PF08352)	Escherichia coli
6	K, L (K, L)	Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL	polymer	226	24737.5	2	UniProt (P16679) Pfam (PF00005)	Escherichia coli	RPnTP synthase subunit PhnL
7	M, N, P, Q (C, D, G, H)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
8	O, R (D, H)	alpha-D-ribose-1,2-cyclic-phosphate-5-phosphate	non-polymer		292.1	2	Chemie (I9X)
9	S, V (I, J)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
10	T, W (I, J)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
11	BA, U, X, Z (L, I, J, K)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
12	AA, Y (L, K)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
13	CA, DA, EA, FA, GA... (A, B, C, D, E...)	water	water		18.0	1171	Chemie (HOH)

Sequence modifications

A, E: 1 - 150 (UniProt: P16685)

PDB	External Database	Details
Leu 85	Gln 85	conflict

C, G: 1 - 354 (UniProt: P16687)

PDB	External Database	Details
Asp 264	Gly 264	conflict
Lys 351	Gln 351	conflict

D, H: 1 - 281 (UniProt: P16688)

PDB	External Database	Details
Leu 103	Val 103	conflict

I, J: 1 - 252 (UniProt: P16678)

PDB	External Database	Details
Glu 253	-	expression tag
Asn 254	-	expression tag
Leu 255	-	expression tag
Tyr 256	-	expression tag
Phe 257	-	expression tag
Gln 258	-	expression tag
Gly 259	-	expression tag
Gln 260	-	expression tag
Phe 261	-	expression tag
Gly 262	-	expression tag
Ser 263	-	expression tag
Trp 264	-	expression tag
Ser 265	-	expression tag
His 266	-	expression tag
Pro 267	-	expression tag
Gln 268	-	expression tag
Phe 269	-	expression tag
Glu 270	-	expression tag
Lys 271	-	expression tag
Gly 272	-	expression tag
Gly 273	-	expression tag
Gly 274	-	expression tag
Ser 275	-	expression tag
Gly 276	-	expression tag
Gly 277	-	expression tag
Gly 278	-	expression tag
Ser 279	-	expression tag
Gly 280	-	expression tag
Gly 281	-	expression tag
Gly 282	-	expression tag
Ser 283	-	expression tag
Trp 284	-	expression tag
Ser 285	-	expression tag
His 286	-	expression tag
Pro 287	-	expression tag
Gln 288	-	expression tag
Phe 289	-	expression tag
Glu 290	-	expression tag
Lys 291	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	330340.7
Non-Polymers*	Number of molecules	16
	Total formula weight	3001.7
All*	Total formula weight	333342.4

*Water molecules are not included.