7YZO

Endonuclease state of the E. coli Mre11-Rad50 (SbcCD) head complex bound to ADP and dsDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	Nuclease SbcCD subunit C	polymer	1048	118851.5	2	UniProt (P13458) Pfam (PF13476) Pfam (PF13558)	Escherichia coli
2	C, D (A, B)	Nuclease SbcCD subunit D	polymer	407	45640.3	2	UniProt (P0AG76) Pfam (PF00149) Pfam (PF12320)	Escherichia coli
3	E (E)	DNA (31-MER)	polymer	120	37099.6	1	GenBank (1825215262)	synthetic construct
4	F (F)	DNA (31-MER)	polymer	120	36970.5	1	GenBank (1825215262)	synthetic construct
5	G, I (C, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
6	H, J (C, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	K, L, M, N (A, B)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)

Sequence modifications

A, B: 1 - 400 (UniProt: P0AG76)

PDB	External Database	Details
Gln 84	His 84	engineered mutation
Gly 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	403053.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1122.8
All*	Total formula weight	404176.5

*Water molecules are not included.