7YZ9

Structure of catalytic domain of Rv1625c bound to nanobody NB4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Adenylate cyclase	polymer	254	28202.8	1	UniProt (P9WQ35) Pfam (PF00211)	Mycobacterium tuberculosis H37Rv	ATP pyrophosphate-lyase,Adenylyl cyclase
2	B (B)	nanobody NB4	polymer	128	13771.2	1		Vicugna pacos
3	C, D, E (A)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
4	F, G (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	H (A)	3'-O-(N-METHYLANTHRANILOYL)-GUANOSINE-5'-TRIPHOSPHATE	non-polymer		656.3	1	Chemie (ONM)
6	I, J (A, B)	water	water		18.0	155	Chemie (HOH)

Sequence modifications

A: 7 - 233 (UniProt: P9WQ35)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Glu -4	-	expression tag
Val -3	-	expression tag
Leu -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Pro 2	-	expression tag
Ser 3	-	expression tag
Gly 4	-	expression tag
His 5	-	expression tag
Met 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	41974.0
Non-Polymers*	Number of molecules	6
	Total formula weight	1005.3
All*	Total formula weight	42979.3

*Water molecules are not included.