7YPN

Crystal structure of transaminase CC1012 mutant M9 complexed with PLP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, D)	Aspartate aminotransferase family protein	polymer	475	52067.4	2	UniProt (A0A318BC23)	Caulobacter sp. D5
2	C, E (B, D)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	D (B)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	F (D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	G, H (B, D)	water	water		18.0	600	Chemie (HOH)

Sequence modifications

B, D: 1 - 465 (UniProt: A0A318BC23)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Phe 25	Gln 25	engineered mutation
Glu 44	Asp 44	engineered mutation
Trp 60	Met 60	engineered mutation
Phe 64	Trp 64	engineered mutation
Phe 138	Tyr 138	engineered mutation
Gln 192	Pro 192	engineered mutation
Ala 266	Ile 266	engineered mutation
Val 377	Thr 377	engineered mutation
Arg 448	Ala 448	engineered mutation
Gly 466	-	expression tag
Glu 467	-	expression tag
Asp 468	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	104134.8
Non-Polymers*	Number of molecules	4
	Total formula weight	662.5
All*	Total formula weight	104797.3

*Water molecules are not included.