7YPB
Cryo-EM structure of Escherichia coli release complex of transcription termination (TTC-release)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1416 | 156537.0 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
4 | E | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
5 | F | DNA (31-MER) | polymer | 31 | 9439.1 | 1 | synthetic construct | ||
6 | G | DNA (31-MER) | polymer | 31 | 9626.2 | 1 | synthetic construct | ||
7 | H | RNA (5'-R(*GP*CP*GP*UP*CP*GP*CP*AP*GP*GP*CP*CP*UP*UP*UP*UP*UP*AP*UP*U)-3') | polymer | 22 | 6949.1 | 1 | synthetic construct | ||
8 | I | RNA (5'-R(*GP*GP*CP*CP*UP*GP*CP*GP*AP*CP*GP*A)-3') | polymer | 12 | 3866.4 | 1 | synthetic construct | ||
9 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
10 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
D: 1 - 1407 (UniProt: P0A8T7)
PDB | External Database | Details |
---|---|---|
Leu 1408 | - | expression tag |
Glu 1409 | - | expression tag |
Val 1410 | - | expression tag |
His 1411 | - | expression tag |
His 1412 | - | expression tag |
His 1413 | - | expression tag |
His 1414 | - | expression tag |
His 1415 | - | expression tag |
His 1416 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 420605.6 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 420760.7 |