7YP7

apo-ADGRG2 coupled to Gs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I,G protein gamma subunit
4	D (N)	Nano-body 35	polymer	160	17352.5	1		Lama glama
5	E (R)	Adhesion G-protein coupled receptor G2	polymer	903	98934.8	1	UniProt (Q8CJ12) Pfam (PF26152) Pfam (PF26574) Pfam (PF01825) Pfam (PF00002)	Mus musculus (house mouse)	G-protein coupled receptor 64,Mouse epididymis-specific protein 6,Me6

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

R: 38 - 891 (UniProt: Q8CJ12)

PDB	External Database	Details
Met 14	-	initiating methionine
Lys 15	-	expression tag
Thr 16	-	expression tag
Ile 17	-	expression tag
Ile 18	-	expression tag
Ala 19	-	expression tag
Leu 20	-	expression tag
Ser 21	-	expression tag
Tyr 22	-	expression tag
Ile 23	-	expression tag
Phe 24	-	expression tag
Cys 25	-	expression tag
Leu 26	-	expression tag
Val 27	-	expression tag
Phe 28	-	expression tag
Ala 29	-	expression tag
Asp 30	-	expression tag
Tyr 31	-	expression tag
Lys 32	-	expression tag
Asp 33	-	expression tag
Asp 34	-	expression tag
Asp 35	-	expression tag
Asp 36	-	expression tag
Lys 37	-	expression tag
Ala 597	His 597	engineered mutation
Ala 599	Thr 599	engineered mutation
Phe 892	-	expression tag
Trp 893	-	expression tag
Phe 894	-	expression tag
Pro 895	-	expression tag
Glu 896	-	expression tag
Lys 897	-	expression tag
Gly 898	-	expression tag
Ala 899	-	expression tag
Ile 900	-	expression tag
Leu 901	-	expression tag
Thr 902	-	expression tag
Asp 903	-	expression tag
Thr 904	-	expression tag
Ser 905	-	expression tag
Val 906	-	expression tag
Lys 907	-	expression tag
Arg 908	-	expression tag
Asn 909	-	expression tag
Asp 910	-	expression tag
Leu 911	-	expression tag
Ser 912	-	expression tag
Ile 913	-	expression tag
Ile 914	-	expression tag
Ser 915	-	expression tag
Gly 916	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	209321.0
All*	Total formula weight	209321.0

*Water molecules are not included.