7YKQ

Crystal structure of a novel alpha/beta hydrolase mutant from thermomonospora curvata in apo form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Triacylglycerol lipase	polymer	269	29440.8	4	UniProt (D1A9G5) Pfam (PF03403)	Thermomonospora curvata DSM 43183
2	E, F, G, H (A, B, C, D)	water	water		18.0	275	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 260 (UniProt: D1A9G5)

PDB	External Database	Details
Asn -8	-	expression tag
Ser -7	-	expression tag
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 13	Ser 42	engineered mutation
Ser 14	Leu 43	engineered mutation
Pro 18	Ala 47	engineered mutation
Thr 41	Arg 70	engineered mutation
Asp 47	Thr 76	engineered mutation
Tyr 92	Leu 121	engineered mutation
Asp 117	Asn 146	engineered mutation
Arg 141	Lys 170	engineered mutation
Arg 142	Ser 171	engineered mutation
Pro 144	Thr 173	engineered mutation
Thr 165	Arg 194	engineered mutation
Asn 175	Leu 204	engineered mutation
Ala 185	Ser 214	engineered mutation
Thr 196	Arg 225	engineered mutation
Gly 205	Asn 234	engineered mutation
Thr 213	Phe 242	engineered mutation
Pro 214	Ser 243	engineered mutation
Ala 224	Ser 253	engineered mutation
Pro 246	Ala 275	engineered mutation
Ser 247	Ile 276	engineered mutation
Glu 252	Asp 281	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117763.2
All*	Total formula weight	117763.2

*Water molecules are not included.