7YK3

Crystal structure of DarTG toxin-antitoxin complex from Mycobacterium tuberculosis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	DNA ADP-ribosyl transferase	polymer	242	26844.4	2	UniProt (O53604) Pfam (PF14487)	Mycobacterium tuberculosis H37Rv	DarT,Toxin DarT,DarT domain-containing protein
2	B, D (B, D)	DNA ADP-ribosyl glycohydrolase	polymer	189	20836.7	2	UniProt (O53605)	Mycobacterium tuberculosis H37Rv	DarG,Antitoxin DarG,Macro domain-containing protein
3	E, F (A, C)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	G, H, I, J (A, B, C, D)	water	water		18.0	329	Chemie (HOH)

Sequence modifications

A, C: 14 - 243 (UniProt: O53604)

PDB	External Database	Details
Met 2	-	initiating methionine
Gly 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Gln 11	-	expression tag
Ala 12	-	expression tag
Ser 13	-	expression tag
Ala 196	Glu 183	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	95362.2
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	95552.1

*Water molecules are not included.