7YJN

Cryo-EM structure of the monomeric atSPT-ORM1 (ORM1-N17A) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Long chain base biosynthesis protein 1	polymer	420	46276.1	1	UniProt (Q94IB8) Pfam (PF00155) In PDB	Arabidopsis thaliana (thale cress)
2	B	Long chain base biosynthesis protein 2a	polymer	489	54359.5	1	UniProt (Q9LSZ9) Pfam (PF00155) In PDB	Arabidopsis thaliana (thale cress)	AtLCB2a,Long chain base biosynthesis protein 2,AtLCB2
3	D	ORMDL family protein	polymer	157	18177.2	1	UniProt (Q9C5I0) Pfam (PF04061) In PDB	Arabidopsis thaliana (thale cress)	atORM1
4	E	Long chain base biosynthesis protein 1	polymer	62	6949.4	1	UniProt (Q94IB8) In PDB	Arabidopsis thaliana (thale cress)	AtLCB1,Protein EMBRYO DEFECTIVE 2779,Protein FUMONISIN B1 RESISTANT 11
5	C	Transmembrane protein, putative (DUF3317)	polymer	77	9442.7	1	UniProt (A8MSB8) Pfam (PF11779) In PDB	Arabidopsis thaliana (thale cress)
6	B	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	1	Chemie (PLP)

Sequence modifications

D: 1 - 157 (UniProt: Q9C5I0)

PDB	External Database	Details
Ala 17	Asn 17	engineered mutation

C: 1 - 56 (UniProt: A8MSB8)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	135204.9
Non-Polymers*	Number of molecules	1
	Total formula weight	247.1
All*	Total formula weight	135452.0

*Water molecules are not included.