7YJ1

Cryo-EM structure of SPT-ORMDL3 (ORMDL3-deltaN2) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Serine palmitoyltransferase 2	polymer	562	63004.2	1	UniProt (O15270) Pfam (PF00155) In PDB	Homo sapiens (human)	Serine-palmitoyl-CoA transferase 2,SPT 2
2	E	Serine palmitoyltransferase 1	polymer	50	5899.0	1	UniProt (O15269) In PDB	Homo sapiens (human)	Long chain base biosynthesis protein 1,LCB 1,Serine-palmitoyl-CoA transferase 1,SPT 1,SPT1
3	D	ORM1-like protein 3	polymer	152	17398.5	1	UniProt (Q8N138) Pfam (PF04061) In PDB	Homo sapiens (human)
4	C	Serine palmitoyltransferase small subunit A	polymer	92	10742.4	1	UniProt (Q969W0) Pfam (PF11779) In PDB	Homo sapiens (human)	Small subunit of serine palmitoyltransferase A
5	A	Serine palmitoyltransferase 1	polymer	423	46925.8	1	UniProt (O15269) Pfam (PF00155) In PDB	Homo sapiens (human)	Long chain base biosynthesis protein 1,LCB 1,Serine-palmitoyl-CoA transferase 1,SPT 1,SPT1
6	B	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	1	Chemie (PLP)

Sequence modifications

D: 3 - 153 (UniProt: Q8N138)

PDB	External Database	Details
Met 2	-	initiating methionine

C: 1 - 71 (UniProt: Q969W0)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	143969.9
Non-Polymers*	Number of molecules	1
	Total formula weight	247.1
All*	Total formula weight	144217.0

*Water molecules are not included.