7YIU
Cryo-EM structure of the C6-ceramide-bound SPT-ORMDL3 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Serine palmitoyltransferase 2 | polymer | 562 | 63004.2 | 1 | UniProt (O15270) Pfam (PF00155) | Homo sapiens (human) | Serine-palmitoyl-CoA transferase 2,SPT 2 |
| 2 | B (E) | Serine palmitoyltransferase 1 | polymer | 50 | 5899.0 | 1 | UniProt (O15269) | Homo sapiens (human) | Long chain base biosynthesis protein 1,LCB 1,Serine-palmitoyl-CoA transferase 1,SPT 1,SPT1 |
| 3 | C (D) | ORM1-like protein 3 | polymer | 153 | 17512.6 | 1 | UniProt (Q8N138) Pfam (PF04061) | Homo sapiens (human) | |
| 4 | D (C) | Serine palmitoyltransferase small subunit A | polymer | 92 | 10742.4 | 1 | UniProt (Q969W0) Pfam (PF11779) | Homo sapiens (human) | Small subunit of serine palmitoyltransferase A,ssSPTa |
| 5 | E (A) | Serine palmitoyltransferase 1 | polymer | 423 | 46925.8 | 1 | UniProt (O15269) Pfam (PF00155) | Homo sapiens (human) | Long chain base biosynthesis protein 1,LCB 1,Serine-palmitoyl-CoA transferase 1,SPT 1,SPT1 |
| 6 | F (B) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) | |||
| 7 | G (D) | N-((E,2S,3R)-1,3-DIHYDROXYOCTADEC-4-EN-2-YL)HEXANAMIDE | non-polymer | 397.6 | 1 | Chemie (6CM) | |||
| 8 | H, I (D) | HEXADECANE | non-polymer | 226.4 | 2 | Chemie (R16) |
Sequence modifications
C: 1 - 71 (UniProt: Q969W0)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Ala -19 | - | expression tag |
| Asp -18 | - | expression tag |
| Tyr -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Asp -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Lys -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Pro -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Arg 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 144084.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1097.7 | |
| All* | Total formula weight | 145181.6 |
