7YHO

CryoEM structure of Arabidopsis ROS1 in complex with TG mismatch dsDNA at 3.3 Angstroms resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sex-determining region Y protein,DNA glycosylase/AP lyase ROS1	polymer	757	86260.9	1	UniProt (Q05066) UniProt (Q9SJQ6) Pfam (PF00505) Pfam (PF15629) Pfam (PF15628) In PDB	Homo sapiens (human)	Testis-determining factor,DEMETER-like protein 1,Protein REPRESSOR OF SILENCING 1,Protein ROS1
2	B	DNA (40-MER)	polymer	40	12395.0	1		Arabidopsis thaliana (thale cress)
3	C	DNA (40-MER)	polymer	40	12244.9	1		Arabidopsis thaliana (thale cress)
4	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 431 - 505 (UniProt: Q05066)

PDB	External Database	Details
Ser 430	-	expression tag
Lys 506	-	linker
Gly 507	-	linker
Gly 508	-	linker
Ser 509	-	linker
Ser 510	-	linker

A: 511 - 835 (UniProt: Q9SJQ6)
A: 836 - 1109 (UniProt: Q9SJQ6)

PDB	External Database	Details
Asn 971	Asp 971	engineered mutation

A: 1110 - 1393 (UniProt: Q9SJQ6)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	110900.7
Non-Polymers*	Number of molecules	1
	Total formula weight	351.6
All*	Total formula weight	111252.3

*Water molecules are not included.