7YFY

Cryo-EM structure of the Mili-piRNA- target ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Piwi-like protein 2	polymer	801	91850.2	1	UniProt (Q8CDG1) Pfam (PF08699) Pfam (PF02170) Pfam (PF02171) In PDB	Mus musculus (house mouse)
2	C	RNA (5'-R(P*CP*CP*AP*UP*GP*UP*UP*GP*AP*UP*GP*GP*UP*AP*A)-3')	polymer	15	4793.9	1		Homo sapiens
3	B	piRNA	polymer	26	8263.0	1		Homo sapiens
4	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 209 - 971 (UniProt: Q8CDG1)

PDB	External Database	Details
Met 171	-	initiating methionine
Asp 172	-	expression tag
Tyr 173	-	expression tag
Lys 174	-	expression tag
Asp 175	-	expression tag
Asp 176	-	expression tag
Asp 177	-	expression tag
Asp 178	-	expression tag
Lys 179	-	expression tag
Gly 180	-	expression tag
Ser 181	-	expression tag
Asp 182	-	expression tag
Tyr 183	-	expression tag
Lys 184	-	expression tag
Asp 185	-	expression tag
Asp 186	-	expression tag
Asp 187	-	expression tag
Asp 188	-	expression tag
Lys 189	-	expression tag
Gly 190	-	expression tag
Ser 191	-	expression tag
Asp 192	-	expression tag
Tyr 193	-	expression tag
Lys 194	-	expression tag
Asp 195	-	expression tag
Asp 196	-	expression tag
Asp 197	-	expression tag
Asp 198	-	expression tag
Lys 199	-	expression tag
Gly 200	-	expression tag
Ser 201	-	expression tag
Glu 202	-	expression tag
Asn 203	-	expression tag
Leu 204	-	expression tag
Tyr 205	-	expression tag
Phe 206	-	expression tag
Gln 207	-	expression tag
Gly 208	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	104907.1
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	104955.7

*Water molecules are not included.