7YFX

Cryo-EM structure of Hili in complex with piRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Piwi-like protein 2	polymer	1011	114404.2	1	UniProt (Q8TC59) Pfam (PF08699) Pfam (PF02170) Pfam (PF02171)	Homo sapiens (human)	Cancer/testis antigen 80,CT80
2	B (B)	piRNA	polymer	26	8263.0	1		Homo sapiens
3	C, D (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 1 - 973 (UniProt: Q8TC59)

PDB	External Database	Details
Met -37	-	initiating methionine
Asp -36	-	expression tag
Tyr -35	-	expression tag
Lys -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Asp -31	-	expression tag
Asp -30	-	expression tag
Lys -29	-	expression tag
Gly -28	-	expression tag
Ser -27	-	expression tag
Asp -26	-	expression tag
Tyr -25	-	expression tag
Lys -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Asp -20	-	expression tag
Lys -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	122667.2
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	122715.8

*Water molecules are not included.