7YFQ

Cryo-EM structure of the EfPiwi (N959K)-piRNA-target ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	piRNA	polymer	25	7957.8	1		Homo sapiens
2	B (C)	RNA (5'-R(*UP*CP*CP*AP*UP*GP*UP*UP*GP*AP*UP*GP*GP*UP*AP*A)-3')	polymer	16	5100.0	1		Homo sapiens
3	C (A)	Piwi	polymer	806	92654.1	1	UniProt (D5MRY8) Pfam (PF02170) Pfam (PF02171)	Ephydatia fluviatilis
4	D, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 220 - 987 (UniProt: D5MRY8)

PDB	External Database	Details
Met 182	-	initiating methionine
Asp 183	-	expression tag
Tyr 184	-	expression tag
Lys 185	-	expression tag
Asp 186	-	expression tag
Asp 187	-	expression tag
Asp 188	-	expression tag
Asp 189	-	expression tag
Lys 190	-	expression tag
Gly 191	-	expression tag
Ser 192	-	expression tag
Asp 193	-	expression tag
Tyr 194	-	expression tag
Lys 195	-	expression tag
Asp 196	-	expression tag
Asp 197	-	expression tag
Asp 198	-	expression tag
Asp 199	-	expression tag
Lys 200	-	expression tag
Gly 201	-	expression tag
Ser 202	-	expression tag
Asp 203	-	expression tag
Tyr 204	-	expression tag
Lys 205	-	expression tag
Asp 206	-	expression tag
Asp 207	-	expression tag
Asp 208	-	expression tag
Asp 209	-	expression tag
Lys 210	-	expression tag
Gly 211	-	expression tag
Ser 212	-	expression tag
Glu 213	-	expression tag
Asn 214	-	expression tag
Leu 215	-	expression tag
Tyr 216	-	expression tag
Phe 217	-	expression tag
Gln 218	-	expression tag
Gly 219	-	expression tag
Lys 959	Asn 959	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	105711.9
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	105760.5

*Water molecules are not included.