7YEL

TR-SFX MmCPDII-DNA complex: 25 us time-point collected in SACLA. Includes 25 us, dark, and extrapolated structure factors

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Deoxyribodipyrimidine photo-lyase	polymer	482	55123.5	2	UniProt (A0A0F8I5V2) Pfam (PF00875) UniProt (by SIFTS) (Q8PYK9) In PDB	Methanosarcina mazei	DNA photolyase
2	C	CPD photolesion containing DNA after repair	polymer	13	3943.6	1		synthetic construct
3	D, F	complementary oligonucleotide to the CPD containing DNA	polymer	14	4305.8	2		synthetic construct
4	E	CPD photolesion containing DNA after repair	polymer	12	3943.6	1		synthetic construct
5	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
6	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7		water	water		18.0	271	Chemie (HOH)

Sequence modifications

A, B: 3 - 462 (UniProt: A0A0F8I5V2)

PDB	External Database	Details
Met -17	-	initiating methionine
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Pro -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
Thr 377	Met 375	engineered mutation
Ala 463	-	expression tag
Leu 464	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	126745.7
Non-Polymers*	Number of molecules	3
	Total formula weight	1667.2
All*	Total formula weight	128412.9

*Water molecules are not included.