7Y9Y

Structure of the Cas7-11-Csx29-guide RNA-target RNA (no PFS) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	RNA (38-MER)	polymer	38	12134.1	1		Desulfonema ishimotonii
2	B (D)	RNA (27-MER)	polymer	27	8703.3	1		Desulfonema ishimotonii
3	C (A)	CRISPR-associated RAMP family protein	polymer	1617	185653.9	1	UniProt (A0A401FT36) Pfam (PF03787)	Desulfonema ishimotonii
4	D (B)	CHAT domain-containing protein	polymer	751	88311.9	1	UniProt (A0A401FT52) Pfam (PF12770)	Desulfonema ishimotonii
5	E, F, G, H (A)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A: 1 - 1601 (UniProt: A0A401FT36)

PDB	External Database	Details
Ala 429	Asp 429	engineered mutation
Ala 654	Asp 654	engineered mutation
Ser 1602	-	expression tag
Ser 1603	-	expression tag
Gly 1604	-	expression tag
Leu 1605	-	expression tag
Val 1606	-	expression tag
Pro 1607	-	expression tag
Arg 1608	-	expression tag
Gly 1609	-	expression tag
Ser 1610	-	expression tag
His 1611	-	expression tag
His 1612	-	expression tag
His 1613	-	expression tag
His 1614	-	expression tag
His 1615	-	expression tag
His 1616	-	expression tag
His 1617	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	294803.2
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	295064.8

*Water molecules are not included.