7Y9P

Xylitol dehydrogenase S96C/S99C/Y102C mutant(thermostabilized form) from Pichia stipitis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Xylitol dehydrogenase	polymer	374	39809.3	1	UniProt (E5G6H4) Pfam (PF08240) Pfam (PF00107) UniProt (by SIFTS) (P22144) In PDB	Scheffersomyces stipitis
2	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6		water	water		18.0	15	Chemie (HOH)

Sequence modifications

A: 2 - 363 (UniProt: E5G6H4)

PDB	External Database	Details
Met -10	-	initiating methionine
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Cys 96	Ser 96	engineered mutation
Cys 99	Ser 99	engineered mutation
Cys 102	Tyr 102	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39809.3
Non-Polymers*	Number of molecules	5
	Total formula weight	425.1
All*	Total formula weight	40234.4

*Water molecules are not included.