7Y85
CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA in complex with TPR-CHAT protease bound to self RNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | RAMP superfamily protein | polymer | 1728 | 198652.7 | 1 | UniProt (A0A0B0EGF3) Pfam (PF03787) In PDB | Candidatus Scalindua brodae | |
| 2 | B | crRNA | polymer | 110 | 35432.1 | 1 | Candidatus Scalindua brodae | ||
| 3 | C | Self RNA target | polymer | 56 | 18097.8 | 1 | Candidatus Scalindua brodae | ||
| 4 | D | CHAT domain protein | polymer | 746 | 85523.1 | 1 | UniProt (A0A0B0EKL4) Pfam (PF12770) In PDB | Candidatus Scalindua brodae | |
| 5 | A | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 6 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 6 - 1722 (UniProt: A0A0B0EGF3)
D: 1 - 716 (UniProt: A0A0B0EKL4)
| PDB | External Database | Details |
|---|---|---|
| Met -5 | - | initiating methionine |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Asn 4 | - | expression tag |
| Asp 5 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 717 | - | expression tag |
| Gly 718 | - | expression tag |
| Ser 719 | - | expression tag |
| Gly 720 | - | expression tag |
| Trp 721 | - | expression tag |
| Ser 722 | - | expression tag |
| His 723 | - | expression tag |
| Pro 724 | - | expression tag |
| Gln 725 | - | expression tag |
| Phe 726 | - | expression tag |
| Glu 727 | - | expression tag |
| Lys 728 | - | expression tag |
| Gly 729 | - | expression tag |
| Gly 730 | - | expression tag |
| Gly 731 | - | expression tag |
| Ser 732 | - | expression tag |
| Gly 733 | - | expression tag |
| Gly 734 | - | expression tag |
| Gly 735 | - | expression tag |
| Ser 736 | - | expression tag |
| Gly 737 | - | expression tag |
| Gly 738 | - | expression tag |
| Trp 739 | - | expression tag |
| Ser 740 | - | expression tag |
| His 741 | - | expression tag |
| Pro 742 | - | expression tag |
| Gln 743 | - | expression tag |
| Phe 744 | - | expression tag |
| Glu 745 | - | expression tag |
| Lys 746 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 337705.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 285.9 | |
| All* | Total formula weight | 337991.7 |
