7Y81

CryoEM structure of type III-E CRISPR Craspase gRAMP-crRNA complex bound to non-self RNA target

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RAMP superfamily protein	polymer	1728	198652.7	1	UniProt (A0A0B0EGF3) Pfam (PF03787) In PDB	Candidatus Scalindua brodae
2	B	crRNA	polymer	110	35432.1	1		Candidatus Scalindua brodae
3	C	Non-self RNA target	polymer	56	18097.8	1		Candidatus Scalindua brodae
4	A	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 6 - 1722 (UniProt: A0A0B0EGF3)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Lys 2	-	expression tag
Ser 3	-	expression tag
Asn 4	-	expression tag
Asp 5	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	252182.6
Non-Polymers*	Number of molecules	5
	Total formula weight	285.9
All*	Total formula weight	252468.5

*Water molecules are not included.