7Y7Z

Cryo-EM structure of human GABA transporter GAT1 bound with tiagabine in NaCl solution in an inward-open state at 3.2 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sodium- and chloride-dependent GABA transporter 1	polymer	632	70895.9	1	UniProt (P30531) Pfam (PF00209)	Homo sapiens (human)	GAT-1,Solute carrier family 6 member 1
2	B (A)	Tiagabine	non-polymer		375.5	1	Chemie (TGI)
3	C, D, E (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)

Sequence modifications

A: 1 - 599 (UniProt: P30531)

PDB	External Database	Details
Leu 600	-	expression tag
Glu 601	-	expression tag
Ser 602	-	expression tag
Gly 603	-	expression tag
Asp 604	-	expression tag
Glu 605	-	expression tag
Val 606	-	expression tag
Asp 607	-	expression tag
Ala 608	-	expression tag
Ser 609	-	expression tag
Gly 610	-	expression tag
Asp 611	-	expression tag
Tyr 612	-	expression tag
Lys 613	-	expression tag
Asp 614	-	expression tag
His 615	-	expression tag
Asp 616	-	expression tag
Gly 617	-	expression tag
Asp 618	-	expression tag
Tyr 619	-	expression tag
Lys 620	-	expression tag
Asp 621	-	expression tag
His 622	-	expression tag
Asp 623	-	expression tag
Ile 624	-	expression tag
Asp 625	-	expression tag
Tyr 626	-	expression tag
Lys 627	-	expression tag
Asp 628	-	expression tag
Asp 629	-	expression tag
Asp 630	-	expression tag
Asp 631	-	expression tag
Lys 632	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	70895.9
Non-Polymers*	Number of molecules	5
	Total formula weight	1074.6
All*	Total formula weight	71970.5

*Water molecules are not included.