7Y59

The cryo-EM structure of human ERAD retro-translocation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (W, X, Y, Z)	Derlin-1	polymer	267	30606.5	4	UniProt (Q9BUN8) Pfam (PF04511)	Homo sapiens (human)	Degradation in endoplasmic reticulum protein 1,DERtrin-1,Der1-like protein 1
2	E, F, G, H, I... (A, B, C, D, E...)	Transitional endoplasmic reticulum ATPase	polymer	787	87546.7	6	UniProt (P55072) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862)	Homo sapiens (human)	TER ATPase,15S Mg(2+)-ATPase p97 subunit,Valosin-containing protein,VCP
3	K, M, O, Q, S... (A, B, C, D, E...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	6	Chemie (ATP)
4	L, N, P, R, T... (A, B, C, D, E...)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	6	Chemie (ADP)

Sequence modifications

W, X, Y, Z: 1 - 251 (UniProt: Q9BUN8)

PDB	External Database	Details
-	Arg 214	deletion
-	Gly 215	deletion
-	Gly 216	deletion
-	Val 217	deletion
-	Ser 218	deletion
-	Gly 219	deletion
-	Phe 220	deletion
-	Gly 221	deletion
-	Val 222	deletion
-	Pro 223	deletion
-	Pro 224	deletion
-	Ala 225	deletion
-	Ser 226	deletion
-	Met 227	deletion
-	Arg 228	deletion
-	Arg 229	deletion
-	Ala 230	deletion
-	Ala 231	deletion
-	Asp 232	deletion
-	Gln 233	deletion
-	Asn 234	deletion
-	Gly 235	deletion
-	Gly 236	deletion
-	Gly 237	deletion
-	Gly 238	deletion
Ala 252	-	expression tag
Ala 253	-	expression tag
Ala 254	-	expression tag
Leu 255	-	expression tag
Glu 256	-	expression tag
Val 257	-	expression tag
Leu 258	-	expression tag
Phe 259	-	expression tag
Gln 260	-	expression tag
Gly 261	-	expression tag
Pro 262	-	expression tag
Ser 263	-	expression tag
Ala 264	-	expression tag
Trp 265	-	expression tag
Ser 266	-	expression tag
His 267	-	expression tag
Pro 268	-	expression tag
Gln 269	-	expression tag
Phe 270	-	expression tag
Glu 271	-	expression tag
Lys 272	-	expression tag
Gly 273	-	expression tag
Gly 274	-	expression tag
Gly 275	-	expression tag
Ser 276	-	expression tag
Gly 277	-	expression tag
Gly 278	-	expression tag
Gly 279	-	expression tag
Ser 280	-	expression tag
Gly 281	-	expression tag
Gly 282	-	expression tag
Ser 283	-	expression tag
Ala 284	-	expression tag
Trp 285	-	expression tag
Ser 286	-	expression tag
His 287	-	expression tag
Pro 288	-	expression tag
Gln 289	-	expression tag
Phe 290	-	expression tag
Glu 291	-	expression tag
Lys 292	-	expression tag

A, B, C, D, E, F: 21 - 806 (UniProt: P55072)

PDB	External Database	Details
Met 20	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	647706.4
Non-Polymers*	Number of molecules	12
	Total formula weight	5606.3
All*	Total formula weight	653312.7

*Water molecules are not included.