7Y47

Crystal structure of bifunctional miltiradiene synthase from selaginella moellendorffii that complexed with GGPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Bifunctional diterpene synthase, chloroplastic	polymer	787	91542.5	1	UniProt (G9MAN7) Pfam (PF01397) Pfam (PF03936) In PDB	Selaginella moellendorffii	Copalyl diphosphate synthase,Miltiradiene synthase,SmMDS,Terpene synthase 4,SmTPS4
2	B	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
3	B	GERANYLGERANYL DIPHOSPHATE	non-polymer		450.4	1	Chemie (GRG)
4		water	water		18.0	231	Chemie (HOH)

Sequence modifications

B: 90 - 867 (UniProt: G9MAN7)

PDB	External Database	Details
Met 89	-	initiating methionine
Pro 136	Ala 136	engineered mutation
Ile 264	Leu 264	engineered mutation
Gly 314	Asp 314	engineered mutation
Thr 409	Pro 409	engineered mutation
Lys 632	Arg 632	engineered mutation
Leu 868	-	expression tag
Glu 869	-	expression tag
His 870	-	expression tag
His 871	-	expression tag
His 872	-	expression tag
His 873	-	expression tag
His 874	-	expression tag
His 875	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	91542.5
Non-Polymers*	Number of molecules	2
	Total formula weight	519.5
All*	Total formula weight	92062.0

*Water molecules are not included.