7Y24

Cryo-EM structure of the octreotide-bound SSTR2-miniGo-scFv16 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	224	25056.6	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	338	37198.7	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	56	6160.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (S)	single Fab chain (svFv16)	polymer	246	26222.2	1		Homo sapiens (human)
5	E (C)	Octreotide	polymer	8	1036.2	1		Homo sapiens
6	F (E)	Somatostatin receptor type 2	polymer	327	36740.6	1	UniProt (P30874) Pfam (PF00001)	Homo sapiens (human)	SS-2-R,SS2-R,SS2R,SST2,SRIF-1

Sequence modifications

A: 5 - 173 (UniProt: P09471)

PDB	External Database	Details
Asp 42	Gly 42	engineered mutation
Asn 43	Glu 43	engineered mutation

A: 182 - 231 (UniProt: P09471)

PDB	External Database	Details
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Gly 180	-	linker
Gly 181	-	linker
Asp 227	Ala 227	engineered mutation
Asp 230	Gly 230	engineered mutation

A: 242 - 354 (UniProt: P09471)

PDB	External Database	Details
Asp 250	Leu 250	engineered mutation
Ala 332	Ile 332	engineered mutation
Ile 335	Val 335	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	132414.4
All*	Total formula weight	132414.4

*Water molecules are not included.