7Y1F

Cryo-EM structure of human k-opioid receptor-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (F)	Dynorphin	polymer	13	1609.0	1	UniProt (by SIFTS) (P01213)	Homo sapiens
3	C (R)	Kappa-type opioid receptor	polymer	460	52317.0	1	UniProt (P41145) Pfam (PF00001)	Homo sapiens (human)	K-OR-1,KOR-1
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (E)	scFv16	polymer	299	32014.8	1		Homo sapiens

Sequence modifications

R: 156 - 460 (UniProt: P41145)

PDB	External Database	Details
Met 1	-	initiating methionine
Lys 2	-	expression tag
Thr 3	-	expression tag
Ile 4	-	expression tag
Ile 5	-	expression tag
Ala 6	-	expression tag
Leu 7	-	expression tag
Ser 8	-	expression tag
Tyr 9	-	expression tag
Ile 10	-	expression tag
Phe 11	-	expression tag
Cys 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Phe 15	-	expression tag
Ala 16	-	expression tag
Asp 17	-	expression tag
Tyr 18	-	expression tag
Lys 19	-	expression tag
Asp 20	-	expression tag
Asp 21	-	expression tag
Asp 22	-	expression tag
Asp 23	-	expression tag
Ala 24	-	expression tag
Lys 25	-	expression tag
Leu 26	-	expression tag
Gln 27	-	expression tag
Thr 28	-	expression tag
Met 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
Glu 40	-	expression tag
Asn 41	-	expression tag
Leu 42	-	expression tag
Tyr 43	-	expression tag
Phe 44	-	expression tag
Gln 45	-	expression tag
Gly 46	-	expression tag
Gly 47	-	expression tag
Thr 48	-	expression tag
Ala 49	-	expression tag
Asp 50	-	expression tag
Leu 51	-	expression tag
Glu 52	-	expression tag
Asp 53	-	expression tag
Asn 54	-	expression tag
Trp 55	-	expression tag
Glu 56	-	expression tag
Thr 57	-	expression tag
Leu 58	-	expression tag
Asn 59	-	expression tag
Asp 60	-	expression tag
Asn 61	-	expression tag
Leu 62	-	expression tag
Lys 63	-	expression tag
Val 64	-	expression tag
Ile 65	-	expression tag
Glu 66	-	expression tag
Lys 67	-	expression tag
Ala 68	-	expression tag
Asp 69	-	expression tag
Asn 70	-	expression tag
Ala 71	-	expression tag
Ala 72	-	expression tag
Gln 73	-	expression tag
Val 74	-	expression tag
Lys 75	-	expression tag
Asp 76	-	expression tag
Ala 77	-	expression tag
Leu 78	-	expression tag
Thr 79	-	expression tag
Lys 80	-	expression tag
Met 81	-	expression tag
Arg 82	-	expression tag
Ala 83	-	expression tag
Ala 84	-	expression tag
Ala 85	-	expression tag
Leu 86	-	expression tag
Asp 87	-	expression tag
Ala 88	-	expression tag
Gln 89	-	expression tag
Lys 90	-	expression tag
Ala 91	-	expression tag
Thr 92	-	expression tag
Pro 93	-	expression tag
Pro 94	-	expression tag
Lys 95	-	expression tag
Leu 96	-	expression tag
Glu 97	-	expression tag
Asp 98	-	expression tag
Lys 99	-	expression tag
Ser 100	-	expression tag
Pro 101	-	expression tag
Asp 102	-	expression tag
Ser 103	-	expression tag
Pro 104	-	expression tag
Glu 105	-	expression tag
Met 106	-	expression tag
Lys 107	-	expression tag
Asp 108	-	expression tag
Phe 109	-	expression tag
Arg 110	-	expression tag
His 111	-	expression tag
Gly 112	-	expression tag
Phe 113	-	expression tag
Asp 114	-	expression tag
Ile 115	-	expression tag
Leu 116	-	expression tag
Val 117	-	expression tag
Gly 118	-	expression tag
Gln 119	-	expression tag
Ile 120	-	expression tag
Asp 121	-	expression tag
Asp 122	-	expression tag
Ala 123	-	expression tag
Leu 124	-	expression tag
Lys 125	-	expression tag
Leu 126	-	expression tag
Ala 127	-	expression tag
Asn 128	-	expression tag
Glu 129	-	expression tag
Gly 130	-	expression tag
Lys 131	-	expression tag
Val 132	-	expression tag
Lys 133	-	expression tag
Glu 134	-	expression tag
Ala 135	-	expression tag
Gln 136	-	expression tag
Ala 137	-	expression tag
Ala 138	-	expression tag
Ala 139	-	expression tag
Glu 140	-	expression tag
Gln 141	-	expression tag
Leu 142	-	expression tag
Lys 143	-	expression tag
Thr 144	-	expression tag
Thr 145	-	expression tag
Arg 146	-	expression tag
Asn 147	-	expression tag
Ala 148	-	expression tag
Tyr 149	-	expression tag
Ile 150	-	expression tag
Gln 151	-	expression tag
Lys 152	-	expression tag
Tyr 153	-	expression tag
Leu 154	-	expression tag
Glu 155	-	expression tag
Leu 237	Ile 135	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	172663.9
All*	Total formula weight	172663.9

*Water molecules are not included.