7Y15
Cryo-EM structure of apo-state MrgD-Gi complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40446.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39461.1 | 1 | UniProt (P62874) Pfam (PF00400) In PDB | Mus musculus (house mouse) | Transducin beta chain 1 |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63213) Pfam (PF00631) In PDB | Mus musculus (house mouse) | G gamma-I |
4 | R | Soluble cytochrome b562,Mas-related G-protein coupled receptor member D | polymer | 446 | 50141.3 | 1 | UniProt (P0ABE7) UniProt (Q8TDS7) Pfam (PF07361) Pfam (PF00001) In PDB | Escherichia coli | Cytochrome b-562,Beta-alanine receptor,G-protein coupled receptor TGR7 |
5 | S | scFV16 | polymer | 296 | 31739.4 | 1 | synthetic construct | ||
6 | R | PALMITIC ACID | non-polymer | 256.4 | 3 | Chemie (PLM) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62874)
R: -100 - 4 (UniProt: P0ABE7)
R: 5 - 321 (UniProt: Q8TDS7)
PDB | External Database | Details |
---|---|---|
Ala 203 | Gly 203 | engineered mutation |
Ala 219 | Thr 219 | engineered mutation |
Gln 288 | Pro 288 | engineered mutation |
Ser 326 | Ala 326 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -15 | - | initiating methionine |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
Glu -6 | - | expression tag |
Asn -5 | - | expression tag |
Leu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Phe -2 | - | expression tag |
Gln -1 | - | expression tag |
Gly 0 | - | expression tag |
Ser 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -124 | - | initiating methionine |
Lys -123 | - | expression tag |
Thr -122 | - | expression tag |
Ile -121 | - | expression tag |
Ile -120 | - | expression tag |
Ala -119 | - | expression tag |
Leu -118 | - | expression tag |
Ser -117 | - | expression tag |
Tyr -116 | - | expression tag |
Ile -115 | - | expression tag |
Phe -114 | - | expression tag |
Cys -113 | - | expression tag |
Leu -112 | - | expression tag |
Val -111 | - | expression tag |
Phe -110 | - | expression tag |
Ala -109 | - | expression tag |
Asp -108 | - | expression tag |
Tyr -107 | - | expression tag |
Lys -106 | - | expression tag |
Asp -105 | - | expression tag |
Asp -104 | - | expression tag |
Asp -103 | - | expression tag |
Asp -102 | - | expression tag |
Lys -101 | - | expression tag |
Trp -94 | Met 29 | engineered mutation |
Ile 1 | His 124 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 169649.0 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 769.3 | |
All* | Total formula weight | 170418.3 |