7Y15

Cryo-EM structure of apo-state MrgD-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40446.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	356	39461.1	1	UniProt (P62874) Pfam (PF00400)	Mus musculus (house mouse)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63213) Pfam (PF00631)	Mus musculus (house mouse)	G gamma-I
4	D (R)	Soluble cytochrome b562,Mas-related G-protein coupled receptor member D	polymer	446	50141.3	1	UniProt (P0ABE7) UniProt (Q8TDS7) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,Beta-alanine receptor,G-protein coupled receptor TGR7
5	E (S)	scFV16	polymer	296	31739.4	1		synthetic construct
6	F, G, H (R)	PALMITIC ACID	non-polymer		256.4	3	Chemie (PLM)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	engineered mutation
Ala 219	Thr 219	engineered mutation
Gln 288	Pro 288	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62874)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

R: -100 - 4 (UniProt: P0ABE7)

PDB	External Database	Details
Met -124	-	initiating methionine
Lys -123	-	expression tag
Thr -122	-	expression tag
Ile -121	-	expression tag
Ile -120	-	expression tag
Ala -119	-	expression tag
Leu -118	-	expression tag
Ser -117	-	expression tag
Tyr -116	-	expression tag
Ile -115	-	expression tag
Phe -114	-	expression tag
Cys -113	-	expression tag
Leu -112	-	expression tag
Val -111	-	expression tag
Phe -110	-	expression tag
Ala -109	-	expression tag
Asp -108	-	expression tag
Tyr -107	-	expression tag
Lys -106	-	expression tag
Asp -105	-	expression tag
Asp -104	-	expression tag
Asp -103	-	expression tag
Asp -102	-	expression tag
Lys -101	-	expression tag
Trp -94	Met 29	engineered mutation
Ile 1	His 124	engineered mutation

R: 5 - 321 (UniProt: Q8TDS7)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	169649.0
Non-Polymers*	Number of molecules	3
	Total formula weight	769.3
All*	Total formula weight	170418.3

*Water molecules are not included.