7Y0T
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with N-imidazolyl-hexanoyl-L-phenylalanyl-L-phenylalanine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B, A | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 465 | 53352.8 | 2 | UniProt (P14779) Pfam (PF00067) In PDB | Priestia megaterium NBRC 15308 = ATCC 14581 | Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3 |
2 | C, D | I7X-PHE-PHE | polymer | 3 | 476.6 | 2 | synthetic construct | ||
3 | B, A | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
4 | water | water | 18.0 | 643 | Chemie (HOH) |
Sequence modifications
B, A: 0 - 455 (UniProt: P14779)
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ala 87 | Phe 88 | engineered mutation |
Leu 456 | - | expression tag |
Glu 457 | - | expression tag |
His 458 | - | expression tag |
His 459 | - | expression tag |
His 460 | - | expression tag |
His 461 | - | expression tag |
His 462 | - | expression tag |
His 463 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 107658.7 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 1233.0 | |
All* | Total formula weight | 108891.6 |