7Y0P
Crystal structure of the P450 BM3 heme domain mutant F87A/T268V/A82T/I263L in complex with N-imidazolyl-hexanoyl-L-phenylalanine, p-cresol and hydroxylamine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B, A | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 465 | 53380.8 | 2 | UniProt (P14779) Pfam (PF00067) In PDB | Priestia megaterium NBRC 15308 = ATCC 14581 | Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3 |
2 | B, A | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
3 | B, A | HYDROXYAMINE | non-polymer | 33.0 | 2 | Chemie (HOA) | |||
4 | B, A | (2S)-2-(6-imidazol-1-ylhexanoylamino)-3-phenyl-propanoic acid | non-polymer | 329.4 | 4 | Chemie (IC6) | |||
5 | B, A | P-CRESOL | non-polymer | 108.1 | 3 | Chemie (PCR) | |||
6 | water | water | 18.0 | 584 | Chemie (HOH) |
Sequence modifications
B, A: 0 - 455 (UniProt: P14779)
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Thr 82 | Ala 83 | engineered mutation |
Ala 87 | Phe 88 | engineered mutation |
Leu 263 | Ile 264 | engineered mutation |
Val 268 | Thr 269 | engineered mutation |
Leu 456 | - | expression tag |
Glu 457 | - | expression tag |
His 458 | - | expression tag |
His 459 | - | expression tag |
His 460 | - | expression tag |
His 461 | - | expression tag |
His 462 | - | expression tag |
His 463 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 106761.6 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 2941.0 | |
All* | Total formula weight | 109702.6 |