7XY9

Cryo-EM structure of secondary alcohol dehydrogenases TbSADH after carrier-free immobilization based on weak intermolecular interactions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	NADP-dependent isopropanol dehydrogenase	polymer	358	38524.7	4	UniProt (P14941) Pfam (PF08240) Pfam (PF00107)	Thermoanaerobacter brockii
2	E, J, M, P (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	F, G, H, I, K... (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
4	Q, R, S, T (A, B, C, D)	water	water		18.0	574	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 352 (UniProt: P14941)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Asn 86	Ile 86	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	154098.8
Non-Polymers*	Number of molecules	12
	Total formula weight	456.1
All*	Total formula weight	154554.9

*Water molecules are not included.